Supplementary Figure 2: Flow cytometry results from seedling leaf of control and putative haploids.
From: One-step genome editing of elite crop germplasm during haploid induction
a) Control diploid showing the 2N peak at 200, and the 4N peak (endoreduplicated or G2 cells) at 400. b) 125-13 was Cas9+ and appears to be a diploid. c) 125-25 was Cas9+ and appears to be a diploid. d) Control haploid showing the 1N peak at 100, and the 2N (endoreduplicated or G2 cells) at 200. e) 125-15 is a haploid (this plant was not edited). f) 125-18 is an edited haploid. g) 125-33 is an edited haploid. h) 125-29 is an edited haploid. All of the samples (in a-h) were run independently two times with similar results.
