Supplementary Figure 7: PCR colony sequencing of GL1 edited haploids.
From: One-step genome editing of elite crop germplasm during haploid induction
Each putative edited target site in the Ler edited haploids’ seedling leaf DNA was amplified by PCR and subcloned followed by sequencing of up to 16 colonies to find the target site mutation and to detect any chimerism. a, 12/15 colonies showed an insertion of a T for 1033-A3. b, 16/16 colonies showed an insertion of an A in 1033-C3. C, 9/9 colonies showed a deletion of a G in 1042-G12. d, 9/9 colonies showed a deletion of a GA at the target site in plant 1042-G10. e, 9/9 colonies showed a deletion of a TG at the target site in plant 1045-E3.
