Supplementary Figure 15: Filtering out severely stressed cells from the single-cell sequencing analysis.
From: Tubuloids derived from human adult kidney and urine for personalized disease modeling
Dissociation-induced stress was quantified using a scoring strategy (see methods) based on the expression of heat shock genes. A score over 0.4 was used to filter out cells that were severely stressed. 192 kidney and 192 tubuloid cells were sequenced in one run and after quality checks (see methods), cells were analyzed for dissociation-induced stress (Kidney 1: n = 41; Kidney 2: n = 43; Tubloid 1: n = 76; Tubloid 2: n = 73). Tukey box plots are used to visualize the distributions. All points of the populations are shown as jittered dots.
