Supplementary Figure 2: Set-up and mutation list of the whole-genome sequencing (WGS) analysis of short-term and long-term tubuloid culture.
From: Tubuloids derived from human adult kidney and urine for personalized disease modeling
A tubuloid line was established from cortical kidney tissue after nephrectomy and tubuloids in passage 2 (P2) and passage 8 (P8) were, along with the matched tissue sample, harvested for WGS. A pie chart with types of mutations and a list of the observed missense mutations in coding regions are included. None of the reported mutations is reported in the COSMIC database or associated with kidney (dys)function. Green boxes indicate the part of the tubuloids that are used for establishing the next passage. Red boxes indicate the samples that were harvested for WGS.
