Supplementary Figure 7: On- and off-target activity measured using modified sgRNAs.
From: Genome-wide target specificity of CRISPR RNA-guided adenine base editors
(a) Mean of base editing frequency when using modified sgRNAs targeted to HEK2, RNF2, EphB4, TYRO3, WEE1, HPRT-exon6, and HPRT-exon8. All data are the results of targeted deep sequencing. (b) Off-target activities measured using modified sgRNAs at the TYRO3 and HPRT-exon8 sites. The red and blue characters indicate the mismatched nucleotides and PAM sequences, respectively. The specificity ratio was calculated using the following formula: specificity of modified sgRNAs (on-target frequency/off-target frequency)/ specificity of conventional sgRNAs (on-target frequency/off-target frequency). Means ± s.e.m. were from three independent experiments.
