Supplementary Figure 10: Specificity of ABE7.10 by combining Sniper ABE7.10 with modified sgRNAs.
From: Genome-wide target specificity of CRISPR RNA-guided adenine base editors
On- and off-target activity and specificity ratio using ABE7.10 and Sniper ABE7.10 with modified sgRNAs at the HPRT-exon6 and HPRT-exon8 sites. The red and blue characters indicate the mismatched nucleotides and PAM sequences, respectively. The specificity ratio was calculated using the following formula: specificity of Sniper ABE7.10 with modified sgRNAs (on-target frequency/off-target frequency)/specificity of ABE7.10 (on-target frequency/off-target frequency). Means ± s.e.m. were from three independent experiments.
