Supplementary Figure 1: Mismatch tolerance of ABE7.10-induced base editing, Cas9-induced indel formation, and BE3-induced base editing in human cells.
From: Genome-wide target specificity of CRISPR RNA-guided adenine base editors
sgRNAs targeting the HEK3 (a) or RNF2 (b) sites have 0 to 4 mismatches compared with the target sequence. The base editing and indel frequencies were measured using targeted deep sequencing. The red and blue characters depict the mismatched nucleotides and the PAM sequences, respectively. Asterisks indicate mismatched sgRNAs whose relative activity (mismatched sgRNA / matched sgRNA) with one enzyme is more than three times higher than that with the other two enzymes. Means ± s.e.m. were from three independent experiments.
