Supplementary Figure 2: Digenome-seq workflow.
From: Genome-wide target specificity of CRISPR RNA-guided adenine base editors
(a) Overview of the Digenome-seq workflow for ABE-mediated Digenome-seq (b) ABE7.10 mediates adenine-to-inosine conversion in one strand and produces a nick in the other strand. hAAG excises inosine to produce an AP site and Endo VIII (DNA glycosylase and AP-lyase) cleaves the AP site. Arrowheads indicate the sites cleaved by the ABE7.10 nickase and hAAG/Endo VIII.
