Supplementary Figure 3: SDS–PAGE analysis of ABE7.10 protein purification using nickel affinity chromatography and heparin bead chromatography. | Nature Biotechnology

Supplementary Figure 3: SDS–PAGE analysis of ABE7.10 protein purification using nickel affinity chromatography and heparin bead chromatography.

From: Genome-wide target specificity of CRISPR RNA-guided adenine base editors

Supplementary Figure 3

M: marker, I(-): cell lysate before IPTG induction, I(+): cell lysate after IPTG induction, S: soluble lysate fraction, IS: insoluble lysate fraction, FT: flow-through, W1, W2: waste after washing, Ni: Ni-NTA agarose beads after elution of bound protein, NE: protein fraction eluted from nickel beads, Hp: Heparin Sepharose 6 Fast Flow affinity resins after elution of bound protein, HE: protein fraction after purification using heparin beads. The red box represents the ABE7.10 protein band. We obtained similar results more than three times when the gel electrophoresis was repeated independently.

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