Supplementary Figure 1: Optimization of Tn5 transposition for dscATAC-seq.
From: Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility
(a) Fraction of reads mapping to the nuclear genome for each of the Tn5 concentrations. The remaining reads map to the mitochondrial genome. Different volumes (2.5–10 μL) of the standard commercial Tn5 (TDE1) are compared against 3 replicates of a custom Tn5 concentration (2.5 μL) optimized for dscATAC-seq for K562 cells. (b) Number of unique reads mapping near transcription start sites (TSS) or (c) distal regulatory elements for the same Tn5 conditions. Center line, median; box limits, first and third quartiles; whiskers, 1.5x interquartile range. All three panels (a-c) show the top 500 cells sorted by library size. (d) Schematic of biochemical process leading to multiple fragments becoming tagged by multiple bead barcodes in the same droplet.
