Supplementary Figure 4: Targeted RNA editing with LEAPER in multiple cell lines.
From: Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs
(a) Western-blot results showing the expression levels of ADAR1, ADAR2 and ADAR3 in indicated human cell lines. β-tubulin was used as a loading control. Data shown is the representative of three independent experiments. ADAR1−/−/ADAR2 represents ADAR1-knockout HEK293T cells overexpressing ADAR2. (b) Relative ADAR protein expression levels normalized by β-tubulin expression. (c) Indicated human cells were transfected with Reporter-1, along with the 71-nt control arRNA (Ctrl RNA71) or with the 71-nt targeting arRNA (arRNA71) followed by FACS analysis. (d) Indicated mouse cell lines were analyzed as described in (c). EGFP+ percentages were normalized by transfection efficiency, which was determined by mCherry+. Error bars in (b, c, d) all indicate the mean ± s.e.m. (n = 3, n represents the number of independent experiments performed in parallel); unpaired two-sided Student’s t-test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.
