Supplementary Figure 8: Evaluation of potential off-targets.
From: Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs
(a) Top, schematic of the highly complementary region between arRNA151-PPIB and the indicated potential off-target sites, which were predicted by searching homologous sequences through NCBI-BLAST. Bottom, Deep sequencing showing the editing rate on the on-target site and all predicted off-target sites of arRNA151-PPIB. Data are presented as the mean ± s.e.m. (n = 3, n represents the number of independent experiments performed in parallel). (b) Schematic of the highly complementary region of arRNA111-FANCC and the indicated potential off-target sequence, which were predicted by searching homologous sequences through NCBI-BLAST. (c) Deep sequencing showing the editing rate on the on-target site and all predicted off-target sites of arRNA111-FANCC. All data are presented as the mean ± s.e.m. (n = 3, n represents the number of independent experiments performed in parallel).
