Supplementary Figure 5: Sorted Tregs can be redirected to kill GBM by BiTE secreted from CAR T cells. | Nature Biotechnology

Supplementary Figure 5: Sorted Tregs can be redirected to kill GBM by BiTE secreted from CAR T cells.

From: CAR-T cells secreting BiTEs circumvent antigen escape without detectable toxicity

Supplementary Figure 5

Bioluminescence-based cytotoxicity assay measuring activity of bystander Tregs against U87, using a transwell system. T cells transduced with either CART-EGFRvIII.BiTE-CD19 (purple) or CART-EGFRvIII.BiTE-EGFR (orange) were cultured in top wells while sorted primary human Tregs (CD4+CD25+CD127dim/-) and U87 targets were placed in bottom wells. The assay was performed in triplicate, n = 3 biologically independent wells (mean + SEM is depicted).

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