Supplementary Figure 3: There is no cross-reactivity between fetal nuclear gene-derived antigens and the Co3 and Cytb 20mers.
From: De novo mutations in mitochondrial DNA of iPSCs produce immunogenic neoepitopes in mice and humans
B/c mice were immunized with B/c ESCs, which were suspended in saline and injected into the thigh muscle. After 5 days, ELISpot assays were performed with recipient splenocytes to test reactivity against 20mers carrying the B6-specific (blue) or B/c-specific (red) amino acid of the SNP at position 6 or 15 (mean ± s.d., quadruplicates of 4 animals per protein, two-tailed Student’s t-test).
