Supplementary Figure 7: Phylogenetic comparison of streptothricin producers and their biosynthetic potential.

(A) A phylogenetic tree was constructed using multilocus sequence analysis with 108 core conserved bacterial genes from 173 actinomycetes, including 42 streptothricin producers. This unrooted phylogeny is displayed as a circular cladogram with a midpoint root. Clade II, clade I and other Streptomyces lineages are highlighted and described by McDonald et al.²⁰ The uncolored strains are other non-Streptomyces actinomycetes. Streptothricin producers are bolded and numbered in both cladogram and heatmap. An enlarged version of this tree is shown as Supplementary Fig. 8. (B) A heatmap of the similarity between biosynthetic genes/domains of 42 streptothricin producers. Streptothricin producers with similar BGCs are scored closer to 1.0 while strains that share few biosynthetic genes other than those for streptothricin biosynthesis are scored close to 0.