Supplementary Figure 4: AAV-Surf library transduced mouse CD8+ T cell surface phenotypes and TCR repertoires before and after adoptive transfer.
(a) Flow cytometry analysis of surface PD-1, Lag3, and Tim-3 expression after transduced with AAV-Vector and AAV-Surf virus. Result from one experiment. (b-c) Flow cytometry analysis of proportion of PD-1+ TILs. ns, non-significant. Data are shown as mean ± s.e.m., with individual data points. The p-values and number of mice used in each group are indicated in the plots and/or in a supplemental excel table. (d) A bar plot of T cell clonal composition from TCR sequencing. Pre injection, mouse CD8+ T cells transduced with AAV-Surf virus and cultured for 5 days; Post injection (TIL), AAV-Surf transduced T cells i.v. injected into GBM-bearing mice and isolated as TILs at day 6 after T cell injection. (e) A ring plot of TCR distribution for T cells before i.v. injection. Top TCR sequences were labeled in the plot. (f) A ring plot of TCR distribution for T cells after i.v. injection (TILs). Top TCR sequences were labeled in the plot.
