Supplementary Figure 6
From: Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs
CRISPR display of Pepper arrays for imaging genomic loci. (a) Schematic presentation of CRISPR display of different pepper arrays for imaging genomic loci. Different Pepper arrays were inserted into the tetraloop, loop2, or both the tetraloop and loop2. (b) Co-labeling of human centromeric-satellites using the modified sgRNA scaffold (red) and dCas9-GFP (green). The nucleus was stained with Hoechst 33342 (blue). Scale bar, 5 μm. (c) Total number of centromeres detected per cell for the three modified sgRNAs. Data represent the mean ± s.d. (N = 10 cells). (d) Quantification of the fluorescence intensities of genomic loci labeled with different Pepper modified sgRNAs. Data represent the mean ± s.d. (n≈400 loci from 10 cells). (e) Dual-color imaging of telomeres and centromeres using Pepper- and Broccoli-modified sgRNA in HeLa cells. Broccoli-DFHBI-1T fluorescence image (original in the insert) was shown with 10×-increased contrast for visualization. Inset, the original image. Scale bar, 10 μm. For b and e, two independent experiments were carried out with similar results.
