Supplementary Figure 7: FokI-EcoCascade editing at an extended panel of genomic target sites. | Nature Biotechnology

Supplementary Figure 7: FokI-EcoCascade editing at an extended panel of genomic target sites.

From: Harnessing type I CRISPR–Cas systems for genome engineering in human cells

Supplementary Figure 7

FokI-EcoCascade editing efficiencies at 49 sites in HEK293 cells after nucleofection with a paired gRNA expression plasmid and a polycistronic plasmid encoding all 5 proteins separate by 2A peptides, n=3 nucleofection replicates per site, bar graph and error bars report the mean and s.d. across nucleofections. All targets contained 30-bp interspacer distances and AAG or ATG PAM sequences.

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