Supplementary Figure 14: Expression and heterologous reconstitution of PseCascade-PseCas3 in HEK293 cells to generate long-range deletions.
From: Harnessing type I CRISPR–Cas systems for genome engineering in human cells
a, Location of eight PseCascade-PseCas3 gRNA target sites in TRAC exon 1. b, HEK293 cells were nucleofected with PseCascade-PseCas3 followed by NGS analysis of edited sites after 4 days. The number of unique deletion classes, relative to an untransfected reference control, were calculated for the eight target sites (x-axis) shown in a, n=2 nucleofection replicates per target site. c, HEK293 cells were nucleofected with PseCascade-PseCas3 targeting site 2 in a followed by analysis of long-range deletions (black) after 5 days, n=2 nucleofection replicates; deletions from one representative replicate are shown. Deletions were enriched using oligo bait probes spanning ~ 2kb surrounding the target site. Deletions < 4 bp were filtered from this display.
