Supplementary Fig. 9: Theoretical assessment of peptide distinguishability from a single nanopore passage.
From: Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore
(a) Number of events per bin per second for LR7 and IR7 (open blue circles) derived from our experimental measurements (Fig. 3d). Gaussian fits, PL(Ib/I0) and PI(Ib/I0), to the experimental data for LR7 and IR7 are shown in black and red, respectively. The shaded area under the PL curve indicates the region of single passage distinguishability of LR7 peptides (see Supplementary Note 1). (b) PL(Ib/I0) and PI(Ib/I0) distributions when the peptide residence time is 200 ms. The shaded area under the PL curve indicates the region of single passage distinguishability. (c) Probability of distinguishing tyrosine (Y) and phenylalanine (F) from a single nanopore passage as a function of the passage duration. (d) Probability of distinguishing leucine (L) from isoleucine (I) when ion counting error is the only noise source.
