Supplementary Fig. 4: Effect of voltage on the discrimination of XR7 peptides.
From: Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore
Histogram of relative residual current Ib/I0 (left) and scatter plot of the blockade duration versus Ib/I0 (right) obtained from nanopore experiment performed using an equimolar mixture of QR7, NR7, TR7, and SR7 peptides under a –25 mV (a), –50 mV (b), –100 mV (c), –150 mV (d) and –200 mV (e) bias applied to the trans compartment. The increase of the voltage magnitude reduces discrimination of the blockade current populations, shifts the Ib/I0 values and decreases the blockade duration. In panels a and b, solid rectangles indicate the location of distributions produced by the QR7 and SR7 peptides; the striped rectangles indicate overlapping distributions of the NR7 and TR7 peptides. In panels c-e, the dashed rectangle indicates the expected location of the QR7, NR7, TR7, and SR7 distributions. The data were acquired in 4 M KCl, 25 mM HEPES buffer, at 7.5 pH and 20.0 ± 0.5oC, and under a –50 mV bias applied to the trans compartment. For each histogram, at least 1000 events were analyzed.
