Supplementary Fig. 14: Imaging depth characterization of MiROM for a polyamide suture embedded in pure fat (lard).
From: Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells
(a) Brightfield image of the phantom array showing two sutures embedded in fat in a measurement cuvette (only one suture was measured). One end of the sutures is attached to the surface of the cuvette while the other end is held by a spacer above the cuvette’s surface, creating an elevation angle of the sutures regarding the surface. (b) Comparison of time arrivals of the OA (PA) signal (envelope) at different positions along the suture’s length, the positions are indicated in c by dashed lines. The legend shows the calculated depth from which the signals arrive. (c) Top view (x-y) maximum amplitude projection of the OA signal, the colored dashed lines correspond to the envelope plots in b. (d) Side view (y-z) maximum amplitude projection of the OA signal. Here, the depth of the OA signal with a SNR of at least 2.4 (~7.6 dB) is indicated. This was found at ~575 µm (L2 in b). Data in a-d are representative of two independent experiments.
