Supplementary Fig. 1: Machine specifications.

a, A representative graphic showing the continuous reduction of the caval pressure until the fluctuation point (pressure <0 mmHg) is reached. After detection of the fluctuation point, the vena cava (VC) target pressure is raised by 1 mmHg. For further details please refer to the Methods. b, Representative illustration of the pO₂ control, where the system automatically adapts FiO₂ of the gas supply to keep a targeted pO₂ in the hepatic artery (HA). c, Representative illustration of the control system adjusting the wash-out rate of CO₂ from the perfusate and thereby, controlling the pH. d, Representative illustration of the automated glucagon infusion. The glucagon supply is integrated as a safety feature to prevent severe hypoglycemia. Glucagon is injected, if the desired glucose level is undershot after spontaneous glucose level recovery was not sufficient. e, Illustration of periodic liver movement with pressurized air. f, Illustration of the current mobile laboratory prototype with internal gas and power supply. Liver4Life refers to the name of our research group. g, Detailed schematics of the perfusion loop. The functions of the integrated components were described in the Methods of the manuscript.