Supplementary Fig. 2: Glucose metabolism in pig livers.
From: An integrated perfusion machine preserves injured human livers for 1 week
The activation of insulin signaling pathway during perfusion for the “hyperglycemic” (n=4 pig livers), “normoglycemic” (n=4 pig livers) and “automated control” (n=4 pig livers) groups. a-b, Insulin induces phosphorylation of Akt and an activation of its signaling pathway. a, P-Akt Western blot analysis and b, quantification. c-d, Glycogen synthase activation depending on insulin application in each study group. P-Akt induces phosphorylation and inactivation of GSK3b, leading to the activation of glycogen synthase. c, P-GSK3b Western blot analysis and d, quantification. e, Glucose level during perfusion for every study group. (hyperglycemic, normoglycemic, automated control). Data reported as mean ± s.d.
