Supplementary Fig. 7: APEC binding to tumor cell surface essential for T cell recognition. | Nature Biotechnology

Supplementary Fig. 7: APEC binding to tumor cell surface essential for T cell recognition.

From: Antibody-mediated delivery of viral epitopes to tumors harnesses CMV-specific T cells for cancer therapy

Supplementary Fig. 7

(a) Flow cytometric analysis of EGF-R and CD20 expression on MDA-MB-231 and JY tumor cell lines. Staining was repeated n=3. (b) Surface binding of APEC is required for antigenic reprogramming and can be inhibited by the pre-treatment with unconjugated antibody (n=3 independent samples). Data represented as mean and error bars represent standard error of the mean. (c) CD20+ tumor cells labelled with cAPEC or rAPEC (350nM) and demonstrating T cell activation only when bound by the anti-CD20 rAPEC. Peptide loaded (1ÎĽM) target cells were used to determine efficacy of T cells (n=3 independent samples). Data represented as mean and error bars represent standard error of the mean.

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