Supplementary Fig. 6: Analysis of empty MHC molecules using HC10 antibody staining.

Colo205 Tumor cells were assayed for the presence and decrease in the amount of empty MHC molecules at the cell surface by HC10 staining. Cells were acid-stripped to remove peptides from surface MHC molecules, lightly fixed or left untreated and incubated with either peptide or APEC before HC10 staining.