Extended Data Fig. 5: Impact of sequencing depth on gene and UMI detection per cell in the PBMC datasets.
From: Systematic comparison of single-cell and single-nucleus RNA-sequencing methods
a–d, The median number of genes (a, b, y-axis) and UMIs (c, d, y-axis) detected per cell at different sequencing depths (x-axis) for low-throughput (a, c) and high-throughput (b, d) methods from PBMC1 (left) and PBMC2 (right). Far right point of each curve: median number of detected genes per cell at full sequencing depth. e, Relation between median number of genes and UMIs per cell in PBMC1 (left) and PBMC2 (right). (n = 1 biologically independent sample for each curve in each plot).
