Supplementary Figure 4: Genomic ancestry analysis of 2018 backcross colonies.

Proportion of Fresno-Clovis ancestry in backcross colonies WB1-CL4 and WB1-CL5 estimated using whole-genome sequencing (~5x) of backcrossed individuals at generations 4 (BC4), 5 (BC5), and 6 (BC6) compared to ‘parental’ WB1-Waco and G1 generations of Fresno-Clovis colonies (CL4 and CL5) . Each dot is an individual mosquito and black horizontal lines indicate the mean across all samples within a generation and colony.