Supplementary Figure 5: 2017 Adult trap data.
In all panels, dashed lines mark the start and end of releases, while the solid line indicates the switch from the backcross colony (WB1-CL1-BC5) to the laboratory colony (WB1-Waco). (a) Number of Wolbachia-males released per day into T3 in 2017. We released a total of 7,316,993 males, of which 1,278,064 were WB1-CL1-BC5 males and 6,038,929 were WB1-Waco. (b) Mean number of males caught per CO2-baited trap in the 2017 treatment (red) and control site (yellow). More backcross males were caught per trap night despite lower release levels. (nT3 = 45, nC2 = 38 independent trap samples per collection day) (c) Mean number of females per CO2-baited trap night in 2017 treatment and control sites (sample sizes same as panel b). After Wolbachia-males were released, female abundance declined in the treatment relative to the control site. (d) Estimated ratio of Wolbachia-males to wild males in treatment site (n = 40 independent trap samples per collection week). (e) Hatch rate of eggs collected in treatment and control sites (nT3 = 81, nC2 = 64 independent trap samples per collection day). (f) Mean number of larvae per twice weekly collection in treatment and control site (sample sizes same as panel e). (g) Cumulative average number of larvae collected over the 2017 field season in treatment and control site (sample sizes same as panel e). (h) Percent suppression in the treatment site relative to the control site based on two-week rolling averages of females per trap (sample sizes same as panel b). For panels b-h, 95% bootstrap confidence intervals are shown as shaded area or bars.
