Extended Data Fig. 8: Absence of colocalization of canonical marker genes for cell types not prioritized by Augur and Fos by RNAscope in situ hybridization.

Schematic indicates imaging location for each marker within the spinal cord. Bottom, proportion of cells expressing Fos from cell types prioritized by Augur (n = 3 cell types) or not prioritized by Augur (n = 6 cell types). Cell types prioritized by Augur are significantly more likely to express Fos after walking with TESS, compared to controls (p = 0.01, two-sided Fisher’s exact test), whereas cell types not prioritized by Augur do not display a statistically significant difference (p = 0.74). Error bars show standard deviation of the sample proportion.