Extended Data Fig. 6: On-target DNA editing activities of NG and VRQR variants of CGBE1 and miniCGBE1 in HEK293T cells.
From: CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
a, Bar plots showing the on-target DNA base editing frequencies induced by NG and VRQR variants of nCas9, CGBE1, and miniCGBE1 using six gRNAs that target AT-rich genomic loci with PAMs that are compatible with SpCas9-NG (NGT) and SpCas9-VRQR (NGAG) variants in HEK293T cells. Editing frequencies of four independent replicates (n = 4) at each base are displayed side-by-side. b, Dot plots representing percentage of alleles that contain an insertion or deletion across the entire protospacer from experiments with NG and VRQR variants of CGBE1 and miniCGBE1 reported in a. Single dots or triangles represent individual replicates (n = 4 independent replicates).
