Fig. 1: Performance data for immunochromatographic LFAs.

a, The second reader’s score (0–6 based on band intensity) is reported for each assay, binned by time after patient-reported symptom onset. Biologically independent samples for each test are as follows: n = 126, Biomedomics; n = 126, Bioperfectus; n = 124, DecomBio; n = 128, DeepBlue; n = 114, Innovita; n = 127, Premier; n = 127, Sure; n = 128, UCP; n = 119, VivaChek; n = 124, Wondfo. The second reader’s score for pre-COVID-19 samples is also displayed (n = 107, Biomedomics; n = 104, Bioperfectus; n = 107, DecomBio; n = 108, DeepBlue; n = 108, Innovita; n = 108, Premier; n = 108, Sure; n = 107, UCP; n = 99, VivaChek; n = 106, Wondfo). For tests with separate IgG and IgM bands, the higher score is reported. Joint IgM/IgG signal is represented by a single band in Wondfo. The lower, dark gray line refers to the positivity threshold (score greater than or equal to 1) used in this study. The upper, light gray line refers to an alternative positivity threshold (score greater than or equal to 2) discussed in the text and Fig. 3. Box spans 25th to 75th percentiles with median indicated by the black bar; whiskers show maximum and minimum value within 1.5× the interquartile range from the box. b, Percent of SARS-CoV-2 RT–PCR-positive samples testing positive by each LFA and ELISA are plotted relative to time after patient-reported symptom onset (n = 126, Biomedomics; n = 126, Bioperfectus; n = 124, DecomBio; n = 128, DeepBlue; n = 114, Innovita; n = 127, Premier; n = 127, Sure; n = 128, UCP; n = 119, VivaChek; n = 124, Wondfo; n = 128, Epitope; n = 128, in-house). The ‘IgM or IgG’ category refers to positivity of either isotype. c, Specificity is plotted for each test using pre-COVID-19 negative control samples (n = 107, Biomedomics; n = 104, Bioperfectus; n = 107, DecomBio; n = 108, DeepBlue; n = 108, Innovita; n = 108, Premier; n = 108, Sure; n = 107, UCP; n = 99, VivaChek; n = 106, Wondfo; n = 108, Epitope; n = 108, in-house). For b and c, all nodes refer to the calculated percent positivity or specificity, respectively. Error bars signify 95% CIs.