Extended Data Fig. 9: Comparison of particle count and defocus effects on final BurrH resolution. | Nature Biotechnology

Extended Data Fig. 9: Comparison of particle count and defocus effects on final BurrH resolution.

From: Molecular goniometers for single-particle cryo-electron microscopy of DNA-binding proteins

Extended Data Fig. 9

a, ResLog plots showing FSC vs. resolution for 3D refinement of BurrH using 1,000 to 68,482 particles. b, Resolution estimates from FSC0.143 vs. particles count. The resolution plateaus at 40k particles, suggesting our resolution is not limited by particle count. c, BurrH resolution vs. maximum micrograph defocus used in 3D reconstruction. d, Resolution estimates from FSC0.143 vs. maximum defocus. The resolution reaches a plateau at 1.5 µm and the addition of higher defocus particles doesn’t comprise the resolution. Inset shows the distribution of BurrH particle defocus in µm.

Back to article page