Extended Data Fig. 10: Filtering particles by estimated rotation angle.
From: Molecular goniometers for single-particle cryo-electron microscopy of DNA-binding proteins
FSC and ResLog plots for BurrH 3D reconstructions in which particles are filtered when their estimated angles from the unfiltered 3D refinement differ from the goniometer goal angle. a, We compared three conditions: No filter (that is, identical to Fig. 6), and two conditions in which particles with assigned rotation angles that differ by >45°, and >90° from the goal angle. b, FSC curves comparing the 3D reconstruction for the three filtering conditions. c, Plot of particle count versus resolution estimate. Filtering reduces the particle count available for the 3D reconstruction, which in turn reduces the final resolution (see Extended Data Fig. 8b). Compared to the unfiltered reconstructions, removing particles in the indicated angle ranges does not improve the final BurrH resolution.
