Extended Data Fig. 10: Perturbations on different sites of the CDKN1A locus caused variant phenotypes.
From: Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs
a, Schematic shows genomic region of CDKN1A and the targeting sites of sgRNAs selected from BARBEKO library (sgRNAStop-1) and newly designed sgRNAs (sgRNAStop2-4 and sgRNASD). b-c, Effects of indicated sgRNAs targeting CDKN1A on cell proliferation in AncBE4max- (b) and Cas9-expressing (c) RPE1 cells. sgRNAAAVS1 served as negative control. Data are presented as the mean ± s.d. of 3 independent experiments. P values represent comparisons with sgRNAAAVS1 at the end point (day 15), calculated using a one-tailed Student’s t-test and adjusted using the Benjamini–Hochberg method, **p < 0.01, ***p < 0.001.
