Extended Data Fig. 3: Information of sgRNAsiBAR and BARBEKO library. | Nature Biotechnology

Extended Data Fig. 3: Information of sgRNAsiBAR and BARBEKO library.

From: Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs

Extended Data Fig. 3

a, Schematic shows the scaffold sequence of sgRNAiBAR, in which 4 iBARs employed in BARBEKO library are highlighted in red. b, Pie chart shows the composition of BARBEKO library that newly designed sgRNAStart and sgRNAs targeting splice sites (sgRNASD and sgRNASA) account for 2.5% and 39.3% respectively, and sgRNAStop introduced from Kuscu et al. account for 58.2%.

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