Fig. 1: Developing a strategy to error-correct barcode and UMI sequences from droplet-based sequencing.
From: Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq
a, Schematic bead and oligonucleotide structure using dimer blocks of nucleotides for Buc-seq. b, Cell barcode-assignment strategy. c, UMI deduplication strategy. d, Simulated data showing the number of barcodes recovered with increasing simulated sequencing error rates. e,f, Simulated data showing the difference and coefficient of variation between the deduplicated UMIs and the ground truth. Correction of the UMI counts was performed using a basic directional network-based approach after accounting for sequencing errors within homodimeric blocks of nucleotides.
