Fig. 6: BoxCar and fractionated DIA.
From: MaxDIA enables library-based and library-free data-independent acquisition proteomics
a, Schedule of libraries and DIA samples. Three different library approaches—single-shot, deep-fractionated and discovery mode—were compared to single-shot, deep-fractionated DIA samples. b, MaxLFQ quantification among three replicates of fractionated BoxCar DIA samples analyzed in discovery DIA mode. All pairwise Pearson correlations are above 0.99. c, Venn diagram-like comparison represented as bar plot between RNA-seq data of HEK cells and three different library methods applied to the fractionated DIA samples. All data have been mapped to gene identifiers d, Histogram of protein identifications mapped to gene identifiers sorted into bins according to log2 RPKM values of the RNA-seq data.
