Extended Data Fig. 1: Organization of immunolabeled dopaminergic fibers.
From: High-throughput mapping of a whole rhesus monkey brain at micrometer resolution
a, MIP of a 200-μm macaque brain slice stained with anti-GFAP antibody (green), an astrocyte marker, and anti-TH antibody (magenta), a marker for dopaminergic neurons. b, The TH channel is displayed individually. The caudate and putamen feature strong background TH signals. Boxed regions are enlarged in (c-h). c, An example color-coded depth image showing dopaminergic axons traveling in GP and pu. d-e, Example images of dopaminergic neurons distributed in PVN (d) and SN (e). f, Dopaminergic fiber bundles are arranged in thin sheets when traveling in icp. A lonely dopaminergic neuron (arrowhead) is captured in GPi and enlarged in the inset. g, Dopaminergic axons project to cortical areas in different patterns. E.g. in the primary motor area (4), dense axons distributed through all the cortical layers, whereas in somatosensory areas (1-2, 3a/b), dopaminergic axons project mainly in superficial layers. white, the gray/white matter boundaries. h-j, Bright dopaminergic fibers could be identified individually in the cortical areas (i) and the white matter (j). Scale bars: (a-b), 5 mm; (c-f), 200 μm; inset of (f), 50 μm; (g-h), 500 μm; (i-j), 100 μm. Acronyms: 1-2, somatosensory areas 1 and 2; 3a/b, somatosensory areas 3a and 3b; 4, primary motor cortex (or F1, agranular frontal area F1); cd, caudate; cis, cingulate sulcus; cs, central sulcus; GP, globus pallidus; GPe, globus pallidus, external segment; GPi, globus pallidus, internal segment; icp, internal capsule, posterior limb; pu, putamen; PVN, paraventricular hypothalamic nucleus; SN, substantia nigra. Experiments were repeated on at least three monkey brain slices, with similar results obtained each time; representative images from a single slice are shown.
