Extended Data Fig. 5: Clonogenic assay confirming differential activity of EGFR kinase inhibitors versus degrader GNE-104.
From: Identifying transcriptional programs underlying cancer drug response with TraCe-seq
(a) Clonogenic assay showing differential anti-growth effects of GNE-104 compared to erlotinib or GNE-069 across four different EGFR-mutant lung cancer cell lines. Scale bar, 1 mm. (b) Quantification of relative viability of the same four EGFR-mutant lung cancer cell lines shown in panel (a) under degrader GNE-104 or non-degrader control GNE-069 treatment relative to erlotinib over 14 days using CellTiter-Glo luminescent cell viability assay. Experiments in (a, b) were run in parallel. Results are representative of 2–4 independent experiments (depending upon the cell line). (c) Clonogenic assay showing that high concentration of free VHL ligand (10 µM) did not affect cellular response to erlotinib or GNE-069 in PC9 cells. The VHL inactive enantiomer was included as a further control. Scale bar, 2 mm. Similar results were observed using two different active VHL ligands.
