Extended Data Fig. 1: Three-dimensional anatomical model of the mouse.
From: Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice
Step 1: The entire mouse was imaged in a computed tomography (CT) scanner. The resulting reconstruction of the entire skeleton of the mouse is shown. Step 2: We acquired high-speed X-ray videographies of a freely behaving mouse, which allowed to capture the extent of the changes in postures of the mouse during activities of daily living. Step 3: The reconstructed mouse skeleton is morphed onto selected X-ray images to quantify the bending radius of the lumbar spinal cord. Step 4: We adapted an antenna to enable magnetic resonance imaging of the lumbar spinal cord, including the visualisation of the posterior roots. We measured the epidural space in these images. Step 5: We transformed these imaging datasets into finite element models of the mouse lumbar spinal cord, including the vertebra, spinal cord and spinal roots. This computer model provides estimates of the suitable dimensions for an implant in the epidural space of the lumbar spinal cord of mice shown in red.
