Extended Data Fig. 6: Metabolically defined monocyte clusters have distinct metabolic activities and regulatory programs. | Nature Biotechnology

Extended Data Fig. 6: Metabolically defined monocyte clusters have distinct metabolic activities and regulatory programs.

From: Integrated analysis of plasma and single immune cells uncovers metabolic changes in individuals with COVID-19

Extended Data Fig. 6

(a) Unsupervised clustering and UMAP of monocytes using expression of only genes involved in metabolic pathways, colored by metabolic cluster and by expression of key metabolic regulator genes. (b) Unsupervised clustering and UMAP of monocytes, colored by all genes-defined cluster and by expression of phenotypic markers. (c) Density map of patient disease severity category per cell overlaid onto UMAP plot: mild/healthy (WHO 0–2), moderate disease (WHO 3–4), and severe disease (WHO 5–7). (d) Heatmap of mean expression of key metabolic regulator genes, phenotypic genes and proteins, and top 10 most active metabolic fluxes for each metabolic cluster in pseudobulk. (e) Metabolic pathway activities of monocyte metabolic clusters in pseudobulk among all patients combined, without inclusion of the dead-cell cluster (metabolic cluster 9).

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