Extended Data Fig. 2: Metabolically defined CD8+ T cell clusters have distinct metabolic activities and regulatory programs. | Nature Biotechnology

Extended Data Fig. 2: Metabolically defined CD8+ T cell clusters have distinct metabolic activities and regulatory programs.

From: Integrated analysis of plasma and single immune cells uncovers metabolic changes in individuals with COVID-19

Extended Data Fig. 2

(a) Unsupervised clustering and UMAP of CD8+ T cells using expression of only genes involved in metabolic pathways, colored by metabolic cluster and by expression of key metabolic regulator genes. (b) Unsupervised clustering and UMAP of CD8+ T cells, colored by all genes-defined cluster and by expression of phenotypic markers. (c) Left: T cell receptor (TCR) clonal expansion per cell overlaid onto UMAP plot. Right: frequency of TCR clonal expansions per metabolic cluster. (d) Density map of patient disease severity category per cell overlaid onto UMAP plot: mild/healthy (WHO 0–2), moderate disease (WHO 3–4), and severe disease (WHO 5–7). (e) Heatmap of mean expression of key metabolic regulator genes, phenotypic genes and proteins, and top 10 most active metabolic fluxes for each metabolic cluster in pseudobulk. (f) Metabolic pathway activities of CD8+ T cell metabolic clusters in pseudobulk among all patients combined, with and without inclusion of proliferative and neoplastic cell clusters (metabolic clusters 4 and 6, respectively).

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