Extended Data Fig. 4: Expression of GRABeCB2.0 has no significant effect on electrically evoked glutamate release in cultured neurons.
From: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
a, Fluorescence microscopy images of neurons expressing Rncp-iGluSnFR (upper) and neurons co-expressing Rncp-iGluSnFR and eCB2.0 (bottom). Similar results were observed for more than 20 neurons. Scale bar, 30 μm. b, Example traces showing the electrical stimulation evoked glutamate signals. c, Pseudocolor change in Rncp-iGluSnFR fluorescence in neurons expressing Rncp-iGluSnFR (upper) and co-expressing Rncp-iGluSnFR and eCB2.0 (bottom) before and after the electrical stimulation. Shown are 25 regions of interest (ROIs) in one culture each. d, Summary of peak Rncp-iGluSnFR ΔF/F0 measured in neurons expressing Rncp-iGluSnFR (upper) or co-expressing Rncp-iGluSnFR and eCB2.0 (bottom); n = 100 ROIs from 4 cultures each. Boxes show the first and third quartiles as well as the median (line), and the whiskers extend to the most extreme data point that is no more than 1.5× the interquartile range from the box. Two-tailed Mann-Whitney test was performed: P = 0.2564. n.s., not significant.
