Extended Data Fig. 6: Spontaneous eCB transients in cultured neurons are sensitive to the CB1R neutral antagonist but not the action potential blocker.
From: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
a, Cumulative transient change in eCB2.0 fluorescence measured during 20 mins of recording in the absence (left) or presence (right) of 1 μM NESS0327. Pseudocolor images were calculated as the average temporal projection subtracted from the maximum temporal projection. Similar results were observed for 3 cultures. Scale bar, 100 μm. b, Summary of the frequency of transient changes in eCB2.0 fluorescence measured in saline (Ctrl) and after NESS0327 application; n = 18 & 18 sessions from 3 cultures with 10-min recording/session. Boxes show the first and third quartiles as well as the median (line), and the whiskers extend to the most extreme data point that is no more than 1.5× the interquartile range from the box. Two-tailed Student’s t test was performed: P = 1.68E-5. c, Cumulative transient change in eCB2.0 fluorescence measured during 20 mins of recording in the absence (left) or presence (right) of 1 μM TTX. Pseudocolor images were calculated as the average temporal projection subtracted from the maximum temporal projection. Similar results were observed for 3 cultures. Scale bar, 100 μm. d, Summary of the frequency of transient changes in eCB2.0 fluorescence measured in saline (Ctrl) and after TTX application; n = 12 & 14 sessions from 3 cultures with 10-min recording/session. Boxes show the first and third quartiles as well as the median (line), and the whiskers extend to the most extreme data point that is no more than 1.5× the interquartile range from the box. Two-tailed Student’s t test was performed: P = 0.5972. ***, p < 0.001; n.s., not significant.
