Extended Data Fig. 8: Detection of eCB signals in acute hippocampal and BLA slices using 2 photon imaging.

a, Schematic diagram depicting the strategy for virus injection in the hippocampal CA1 region, followed by the preparation of acute slices for electrical stimulation and 2-photon imaging. b, (Left) fluorescence image of eCB2.0 expressed in the hippocampal CA1 region, showing the position of the stimulating electrode. (Right) pseudocolor images showing the change in eCB2.0 fluorescence at baseline and after 10 or 50 pulses applied at 20 Hz. The dashed circle shows the ROI for quantification. Similar results were observed for 5 slices. Scale bar, 100 μm. c, Representative traces and summary of the peak change in eCB2.0 fluorescence evoked by electrical pulses applied at the indicated frequencies; n = 5 slices, mean ± s.e.m. d, Time course of the change in eCB2.0 fluorescence; where indicated, AEA and AM251 were applied. e, Representative traces of the change in eCB2.0 fluorescence evoked by electrical stimulation in the absence and presence of AM251. f, Schematic diagram depicting the strategy for virus injection in the BLA region, followed by the preparation of acute slices for electrical stimulation and 2-photon imaging. g, Pseudocolor images showing the change in eCB2.0 fluorescence after 20, 50 or 100 pulses applied at 20 Hz. Similar results were observed for 3 slices. Scale bar, 100 μm. h, Traces of eCB2.0 fluorescence evoked by electrical pulses applied at the indicated frequencies. i, Representative pseudocolor image, trace, and summary of peak change in eCB2.0 fluorescence upon 75 mM K⁺ ACSF perfusion. Scale bar, 100 μm. n = 3 slices, mean ± s.e.m.

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