Extended Data Fig. 9: Expression of GRABeCB sensors has minimal effect on animal behaviors.
From: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
a, Fluorescence images of coronal slices prepared from mice expressing GFP or GRABeCB2.0 in BLA. Similar results were observed for 6 mice. Scale bar, 1 mm. b, Schematic diagrams showing the open field test (OFT) and the elevated plus maze test (EPMT). c, Quantification of behavioral parameters in the OFT. n = 6 mice, mean ± s.e.m. Two-tailed Student’s t tests were performed: P = 0.2084, 0.8737, 0.5858 and 0.4464. d, Quantification of behavioral parameters in the EPMT. n = 6 mice, mean ± s.e.m. Two-tailed Student’s t tests were performed: P = 0.2912, 0.5377, 0.6007, 0.3386, 0.3748, 0.4958 and 0.1411. e, Schematic diagram showing the fear conditioning test. f, Quantification of freezing behavior before, during and after conditioning. n = 6 mice, mean ± s.e.m. Two-way ANOVA test was performed: P = 0.3799 (between two animal groups during conditioning); two-tailed Student’s t tests were performed: P = 0.3297 and 0.8669 (during retrieval). g, Quantification of averaged speed, running speed and averaged distance in control, eCB2.0 and eCBmut expressing mice; n = 19, 8 and 6 mice. Boxes show the first and third quartiles as well as the median (line), and the whiskers extend to the most extreme data point that is no more than 1.5× the interquartile range from the box. One-way ANOVA tests were performed: P = 0.9017, 0.0681 and 0.4197. n.s., not significant.
