Extended Data Fig. 1: Strategy for optimizing and screening the GRABeCB sensors.
From: A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
a, A flowchart showing the development process of the eCB2.0 sensor. Responses to 10 μM 2-AG of candidate sensors were shown alongside each step. b, Schematic diagram depicting the structure of the GRABeCB2.0 sensor. The IgK leader sequence and the sequence derived from GRABNE are shown. c, Amino acids sequence of the eCB2.0 sensor. The phenylalanine residue at position 1772.64 in the CB1R was mutated to an alanine to generate the eCBmut sensor (indicated by the gray box). Note that the numbering used in the figure corresponds to the start of the IgK leader sequence.
