Fig. 3: T1 and T3 cells efficiently kill leukemia cells in vivo.
From: T cells targeted to TdT kill leukemic lymphoblasts while sparing normal lymphocytes
a, Viable TdTposHLA-A2pos NALM-6 and BV173 cells after 48 h of coculture with T1 and T3 cells (E/T ratio, 1/1), in percentage of corresponding numbers following treatment with mock-transduced T cells as quantified by flow cytometry. Data points represent technical replicates in one experiment representative of three performed. b, Experimental overview of BV173 and NALM-6 in vivo models. BLI (c) and quantification (d) of BV173-bearing mice 1 day before and 21 days after treatment with human T cells transduced with 1G4, T1 or T3, or left untreated. Survival analysis (e) and numbers (f) of TCR-transduced CD8+ T cells μl–1 blood at indicated time points in the aforementioned experimental groups. BLI (g) and quantification (h) of NALM-6-bearing mice 1 day before and 14 days after treatment with human T cells transduced with 1G4, T3, or left untreated. Survival analysis (i) and numbers (j) of TCR-transduced CD8+ T cells μl–1 blood at indicated time points in the aforementioned experimental groups. d,e,h,i, Data are pooled from two independent experiments: untreated, n = 10 (BV173) or n = 11 (NALM-6); 1G4, n = 7 (BV173) or n = 9 (NALM-6); T1, n = 8 (BV173); T3, n = 9 (BV173) or n = 11 (NALM-6). d,h, Box plots showing interquartile range (25th–75th percentile) with central bar indicating the median and whiskers indicating the range. Dots represent individual mice and data analyzed by one-way ANOVA with adjustment for multiple comparisons with Tukey’s post-test. e,i, Survival analysis performed by two-sided log-rank (Mantel–Cox) test. f,j, Data shown are from one representative experiment out of two performed. n = 5–6 mice per group. Connecting lines, mean; dots, individual mice.
