Extended Data Fig. 6: Dual-color live-cell imaging of clathrin and actin by Sparse SD-SIM (Supplementary Video 16).
From: Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy

(a,b) Color temporal projections of CCPs (a) and the actin filament network (b) recorded by SD-SIM (left) and Sparse SD-SIM (right) for 16.75 minutes at 5 s intervals. (c) CCPs (cyan) and the cortical actin cytoskeleton (magenta) in a COS-7 cell captured by Sparse SD-SIM. (d) Montages of the boxed region in (c) at five-time points are shown at a magnified scale; the first image observed under SD-SIM appears at the top left corner for comparison. It can be observed that a CCP docks stably at the junction of two actin filaments and then disappears from the focal plane as these neighboring filaments merge. Experiments were repeated five times independently with similar results. Scale bars: (a-c) 4 μm; (d) 500 nm.